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tlr9 agonist class c cpg odn  (InvivoGen)


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    InvivoGen tlr9 agonist class c cpg odn
    Frequency of tonsillar pDCs positively correlates with the expression of mRNAs encoding APRIL, BAFF and <t>TLR9</t> in tonsillar MNCs from patients with IgAN. Correlation analysis was performed between the frequency of tonsillar pDCs and gene expression in tonsillar MNCs from patients with IgAN ( n = 10, red dots) and CT ( n = 9, black dots). ( A ) Correlation between the percentage of tonsillar pDCs and the expression of TLR9 in tonsillar MNCs from patients with IgAN and CT. ( B ) Correlation between the percentage of tonsillar pDCs and the expression of IL-6 in tonsillar MNCs from patients with IgAN and CT. ( C ) Correlation between the percentage of tonsillar pDCs and the expression of BAFF in tonsillar MNCs from patients with IgAN and CT. ( D ) Correlation between the percentage of tonsillar pDCs and the expression of APRIL in tonsillar MNCs from patients with IgAN and CT. Spearman’s correlation coefficients (r) and P -values are shown in each panel. * P ≤ .05.
    Tlr9 Agonist Class C Cpg Odn, supplied by InvivoGen, used in various techniques. Bioz Stars score: 97/100, based on 413 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cpg+odn+class+2395/pmc13130947-83-1-8?v=InvivoGen
    Average 97 stars, based on 413 article reviews
    tlr9 agonist class c cpg odn - by Bioz Stars, 2026-07
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    1) Product Images from "Plasmacytoid dendritic cells modulate the production of mucosal IgA in IgA nephropathy"

    Article Title: Plasmacytoid dendritic cells modulate the production of mucosal IgA in IgA nephropathy

    Journal: Nephrology Dialysis Transplantation

    doi: 10.1093/ndt/gfaf216

    Frequency of tonsillar pDCs positively correlates with the expression of mRNAs encoding APRIL, BAFF and TLR9 in tonsillar MNCs from patients with IgAN. Correlation analysis was performed between the frequency of tonsillar pDCs and gene expression in tonsillar MNCs from patients with IgAN ( n = 10, red dots) and CT ( n = 9, black dots). ( A ) Correlation between the percentage of tonsillar pDCs and the expression of TLR9 in tonsillar MNCs from patients with IgAN and CT. ( B ) Correlation between the percentage of tonsillar pDCs and the expression of IL-6 in tonsillar MNCs from patients with IgAN and CT. ( C ) Correlation between the percentage of tonsillar pDCs and the expression of BAFF in tonsillar MNCs from patients with IgAN and CT. ( D ) Correlation between the percentage of tonsillar pDCs and the expression of APRIL in tonsillar MNCs from patients with IgAN and CT. Spearman’s correlation coefficients (r) and P -values are shown in each panel. * P ≤ .05.
    Figure Legend Snippet: Frequency of tonsillar pDCs positively correlates with the expression of mRNAs encoding APRIL, BAFF and TLR9 in tonsillar MNCs from patients with IgAN. Correlation analysis was performed between the frequency of tonsillar pDCs and gene expression in tonsillar MNCs from patients with IgAN ( n = 10, red dots) and CT ( n = 9, black dots). ( A ) Correlation between the percentage of tonsillar pDCs and the expression of TLR9 in tonsillar MNCs from patients with IgAN and CT. ( B ) Correlation between the percentage of tonsillar pDCs and the expression of IL-6 in tonsillar MNCs from patients with IgAN and CT. ( C ) Correlation between the percentage of tonsillar pDCs and the expression of BAFF in tonsillar MNCs from patients with IgAN and CT. ( D ) Correlation between the percentage of tonsillar pDCs and the expression of APRIL in tonsillar MNCs from patients with IgAN and CT. Spearman’s correlation coefficients (r) and P -values are shown in each panel. * P ≤ .05.

    Techniques Used: Expressing, Gene Expression

    Ratio of Gd-IgA1/IgA levels in culture supernatants of tonsillar MNCs from patients with IgAN correlates with the percentage of pDCs and the expression of APRIL in tonsillar MNCs. Association between Gd-IgA1/IgA levels and tonsillar pDC frequency or gene expression in MNCs from patients with IgAN ( n = 7, red dots) and CT ( n = 8, black dots). ( A ) The ratios of Gd-IgA1/IgA in culture supernatants of tonsillar MNCs from patients with IgAN or CT. ( B ) Correlation between the Gd-IgA1/IgA ratio and the percentage of tonsillar pDCs in MNCs from patients with IgAN and CT. ( C ) Correlation between the Gd-IgA1/IgA ratio and the expression of APRIL in MNCs from patients with IgAN and CT. ( D ) Correlation between the Gd-IgA1/IgA ratio and the expression of BAFF in MNCs from patients with IgAN and CT. ( E ) Association between the APRIL and TLR9 expression levels in tonsillar MNCs from patients with IgAN and CT. Correlations were assessed using Spearman’s correlation coefficients (r) with P -values shown in each panel. ns, not significant; * P ≤ .05.
    Figure Legend Snippet: Ratio of Gd-IgA1/IgA levels in culture supernatants of tonsillar MNCs from patients with IgAN correlates with the percentage of pDCs and the expression of APRIL in tonsillar MNCs. Association between Gd-IgA1/IgA levels and tonsillar pDC frequency or gene expression in MNCs from patients with IgAN ( n = 7, red dots) and CT ( n = 8, black dots). ( A ) The ratios of Gd-IgA1/IgA in culture supernatants of tonsillar MNCs from patients with IgAN or CT. ( B ) Correlation between the Gd-IgA1/IgA ratio and the percentage of tonsillar pDCs in MNCs from patients with IgAN and CT. ( C ) Correlation between the Gd-IgA1/IgA ratio and the expression of APRIL in MNCs from patients with IgAN and CT. ( D ) Correlation between the Gd-IgA1/IgA ratio and the expression of BAFF in MNCs from patients with IgAN and CT. ( E ) Association between the APRIL and TLR9 expression levels in tonsillar MNCs from patients with IgAN and CT. Correlations were assessed using Spearman’s correlation coefficients (r) with P -values shown in each panel. ns, not significant; * P ≤ .05.

    Techniques Used: Expressing, Gene Expression



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    Frequency of tonsillar pDCs positively correlates with the expression of mRNAs encoding APRIL, BAFF and <t>TLR9</t> in tonsillar MNCs from patients with IgAN. Correlation analysis was performed between the frequency of tonsillar pDCs and gene expression in tonsillar MNCs from patients with IgAN ( n = 10, red dots) and CT ( n = 9, black dots). ( A ) Correlation between the percentage of tonsillar pDCs and the expression of TLR9 in tonsillar MNCs from patients with IgAN and CT. ( B ) Correlation between the percentage of tonsillar pDCs and the expression of IL-6 in tonsillar MNCs from patients with IgAN and CT. ( C ) Correlation between the percentage of tonsillar pDCs and the expression of BAFF in tonsillar MNCs from patients with IgAN and CT. ( D ) Correlation between the percentage of tonsillar pDCs and the expression of APRIL in tonsillar MNCs from patients with IgAN and CT. Spearman’s correlation coefficients (r) and P -values are shown in each panel. * P ≤ .05.
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    Frequency of tonsillar pDCs positively correlates with the expression of mRNAs encoding APRIL, BAFF and <t>TLR9</t> in tonsillar MNCs from patients with IgAN. Correlation analysis was performed between the frequency of tonsillar pDCs and gene expression in tonsillar MNCs from patients with IgAN ( n = 10, red dots) and CT ( n = 9, black dots). ( A ) Correlation between the percentage of tonsillar pDCs and the expression of TLR9 in tonsillar MNCs from patients with IgAN and CT. ( B ) Correlation between the percentage of tonsillar pDCs and the expression of IL-6 in tonsillar MNCs from patients with IgAN and CT. ( C ) Correlation between the percentage of tonsillar pDCs and the expression of BAFF in tonsillar MNCs from patients with IgAN and CT. ( D ) Correlation between the percentage of tonsillar pDCs and the expression of APRIL in tonsillar MNCs from patients with IgAN and CT. Spearman’s correlation coefficients (r) and P -values are shown in each panel. * P ≤ .05.
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    Image Search Results


    Frequency of tonsillar pDCs positively correlates with the expression of mRNAs encoding APRIL, BAFF and TLR9 in tonsillar MNCs from patients with IgAN. Correlation analysis was performed between the frequency of tonsillar pDCs and gene expression in tonsillar MNCs from patients with IgAN ( n = 10, red dots) and CT ( n = 9, black dots). ( A ) Correlation between the percentage of tonsillar pDCs and the expression of TLR9 in tonsillar MNCs from patients with IgAN and CT. ( B ) Correlation between the percentage of tonsillar pDCs and the expression of IL-6 in tonsillar MNCs from patients with IgAN and CT. ( C ) Correlation between the percentage of tonsillar pDCs and the expression of BAFF in tonsillar MNCs from patients with IgAN and CT. ( D ) Correlation between the percentage of tonsillar pDCs and the expression of APRIL in tonsillar MNCs from patients with IgAN and CT. Spearman’s correlation coefficients (r) and P -values are shown in each panel. * P ≤ .05.

    Journal: Nephrology Dialysis Transplantation

    Article Title: Plasmacytoid dendritic cells modulate the production of mucosal IgA in IgA nephropathy

    doi: 10.1093/ndt/gfaf216

    Figure Lengend Snippet: Frequency of tonsillar pDCs positively correlates with the expression of mRNAs encoding APRIL, BAFF and TLR9 in tonsillar MNCs from patients with IgAN. Correlation analysis was performed between the frequency of tonsillar pDCs and gene expression in tonsillar MNCs from patients with IgAN ( n = 10, red dots) and CT ( n = 9, black dots). ( A ) Correlation between the percentage of tonsillar pDCs and the expression of TLR9 in tonsillar MNCs from patients with IgAN and CT. ( B ) Correlation between the percentage of tonsillar pDCs and the expression of IL-6 in tonsillar MNCs from patients with IgAN and CT. ( C ) Correlation between the percentage of tonsillar pDCs and the expression of BAFF in tonsillar MNCs from patients with IgAN and CT. ( D ) Correlation between the percentage of tonsillar pDCs and the expression of APRIL in tonsillar MNCs from patients with IgAN and CT. Spearman’s correlation coefficients (r) and P -values are shown in each panel. * P ≤ .05.

    Article Snippet: The TLR9 agonist class C CpG-ODN (ODN 2395, InvivoGen), which stimulates pDCs and B cells [ ], was intranasally administered to each group of mice every 2 days for 3 weeks.

    Techniques: Expressing, Gene Expression

    Ratio of Gd-IgA1/IgA levels in culture supernatants of tonsillar MNCs from patients with IgAN correlates with the percentage of pDCs and the expression of APRIL in tonsillar MNCs. Association between Gd-IgA1/IgA levels and tonsillar pDC frequency or gene expression in MNCs from patients with IgAN ( n = 7, red dots) and CT ( n = 8, black dots). ( A ) The ratios of Gd-IgA1/IgA in culture supernatants of tonsillar MNCs from patients with IgAN or CT. ( B ) Correlation between the Gd-IgA1/IgA ratio and the percentage of tonsillar pDCs in MNCs from patients with IgAN and CT. ( C ) Correlation between the Gd-IgA1/IgA ratio and the expression of APRIL in MNCs from patients with IgAN and CT. ( D ) Correlation between the Gd-IgA1/IgA ratio and the expression of BAFF in MNCs from patients with IgAN and CT. ( E ) Association between the APRIL and TLR9 expression levels in tonsillar MNCs from patients with IgAN and CT. Correlations were assessed using Spearman’s correlation coefficients (r) with P -values shown in each panel. ns, not significant; * P ≤ .05.

    Journal: Nephrology Dialysis Transplantation

    Article Title: Plasmacytoid dendritic cells modulate the production of mucosal IgA in IgA nephropathy

    doi: 10.1093/ndt/gfaf216

    Figure Lengend Snippet: Ratio of Gd-IgA1/IgA levels in culture supernatants of tonsillar MNCs from patients with IgAN correlates with the percentage of pDCs and the expression of APRIL in tonsillar MNCs. Association between Gd-IgA1/IgA levels and tonsillar pDC frequency or gene expression in MNCs from patients with IgAN ( n = 7, red dots) and CT ( n = 8, black dots). ( A ) The ratios of Gd-IgA1/IgA in culture supernatants of tonsillar MNCs from patients with IgAN or CT. ( B ) Correlation between the Gd-IgA1/IgA ratio and the percentage of tonsillar pDCs in MNCs from patients with IgAN and CT. ( C ) Correlation between the Gd-IgA1/IgA ratio and the expression of APRIL in MNCs from patients with IgAN and CT. ( D ) Correlation between the Gd-IgA1/IgA ratio and the expression of BAFF in MNCs from patients with IgAN and CT. ( E ) Association between the APRIL and TLR9 expression levels in tonsillar MNCs from patients with IgAN and CT. Correlations were assessed using Spearman’s correlation coefficients (r) with P -values shown in each panel. ns, not significant; * P ≤ .05.

    Article Snippet: The TLR9 agonist class C CpG-ODN (ODN 2395, InvivoGen), which stimulates pDCs and B cells [ ], was intranasally administered to each group of mice every 2 days for 3 weeks.

    Techniques: Expressing, Gene Expression

    FIGURE 1 Innate immune responses to AAV serotypes in human blood. (A–D) Whole blood from healthy human donors was stimulated with AAV6-GFP or AAV9-GFP at 0,3,10, and 30 E10 vg or TLR9 agonist CpGC. Scatter plots of secreted IFN-a and IL-6 in response to AAV6-GFP (A, B) or AAV9-GFP (C, D). Data in (A, C) were combined from two independent experiments (n=8 donors). Data in (B, D) represent one independent experiment with (n=4). (E, F) Whole blood from healthy human donors was stimulated with mammalian cell-derived AAV9-HEK carrying a human transgene at 10 E10 vg or TLR9 agonist CpGC. Scatter plots of secreted IFN-a (E) and IL-6 (F) in response to AAV9-HEK. P-value was determined based on a one-way ANOVA nonparametric multiple comparison test with Dunn method, *P <0.05, and **P <0.01. “Unstim” indicates unstimulated.

    Journal: Frontiers in immunology

    Article Title: Essential role of pre-existing humoral immunity in TLR9-mediated type I IFN response to recombinant AAV vectors in human whole blood.

    doi: 10.3389/fimmu.2024.1354055

    Figure Lengend Snippet: FIGURE 1 Innate immune responses to AAV serotypes in human blood. (A–D) Whole blood from healthy human donors was stimulated with AAV6-GFP or AAV9-GFP at 0,3,10, and 30 E10 vg or TLR9 agonist CpGC. Scatter plots of secreted IFN-a and IL-6 in response to AAV6-GFP (A, B) or AAV9-GFP (C, D). Data in (A, C) were combined from two independent experiments (n=8 donors). Data in (B, D) represent one independent experiment with (n=4). (E, F) Whole blood from healthy human donors was stimulated with mammalian cell-derived AAV9-HEK carrying a human transgene at 10 E10 vg or TLR9 agonist CpGC. Scatter plots of secreted IFN-a (E) and IL-6 (F) in response to AAV9-HEK. P-value was determined based on a one-way ANOVA nonparametric multiple comparison test with Dunn method, *P <0.05, and **P <0.01. “Unstim” indicates unstimulated.

    Article Snippet: Blood samples were also stimulated with 5mg/ml of a Class C CpG oligonucleotide (CpGC) (InvivoGen, ODN2395, Cat# tlrl-2395) as a positive control for TLR9 activation.

    Techniques: Derivative Assay, Comparison